Primary Cell RNA
Cell Applications Inc. provides the highest quality RNA available. A variety of Total RNA's are prepared from primary cells and tissues from human and animal using a modified guanidium thiocyanate method. Purified RNA is ready for use in various downstream applications, including microarray hybridization, PCR cDNA synthesis and RT-PCR analysis. RNA is offered at 10 μg and 25 μg per vial at concentration of 1 mg/ml, and are ready for use in downstream applications.
Stringent quality control tests were performed on each preparation to confirm the integrity and stability of RNA:
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Distinct 28S and 18S ribosomal RNA were shown on 1% denaturing gel before and after 37oC incubation.
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No visible Genomic DNA band of 23 kb was detected.
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No genomic DNA contamination was detected following PCR amplification using house-keeping gene G3PDH primers.
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A 2.2 kb product from mRNA was detected following RT-PCR amplification using human heat shock gene Hsp60 primers.
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A discrete band of 2 kb was detected upon hybridization with a human beta-actin cDNA probe in Northern Blot analysis.
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Primers were designed to detect mRNA for genes of interest to confirm the phenotype of the cells.
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CADMEC RNA, adult 10mg
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Human Dermal Microvascular Endothelial Cells RNA, adult Läs mer -
CADMEC RNA, adult 25mg
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Human Dermal Microvascular Endothelial Cells RNA, adult Läs mer -
CADMEC RNA, neonatal 10 mg
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Human Dermal Microvascular Endothelial Cells RNA, neonatal Läs mer -
CADMEC RNA, neonatal 25 mg
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Human Dermal Microvascular Endothelial Cells RNA, neonatal Läs mer -
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Differentiated HOb RNA, fetal 10mg
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Human Osteoblast, Differentiated RNA, fetal Läs mer -
Differentiated HOb RNA, fetal 25mg
Beställningsvara
Human Osteoblast, Differentiated RNA, fetal Läs mer -
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