Tissue RNA

Cell Applications Inc. guarantees to provide the highest quality RNA available. A variety of Total RNA's are prepared from primary cells and tissues from normal human and animal using a modified guanidium thiocyanate method. RNA is DNAse treated to remove contaminating genomic DNA and undergoes stringent quality control tests. Purified RNA is ready for use in convenient 50 µg and 250 µg sizes for various downstream applications, including microarray hybridization, PCR cDNA synthesis and RT-PCR analysis.

Stringent quality control tests were performed on each preparation to confirm the integrity and stability of RNA:

• Distinct 28S and 18S ribosomal RNA were shown on 1% denaturing gel before and after 37^oC  incubation.
• No visible Genomic DNA band of 23 kb was detected.
• No genomic DNA contamination was detected following PCR amplification using house-keeping gene G3PDH primers.
• A 2.2 kb product from mRNA was detected following RT-PCR amplification using human heat shock gene Hsp60 primers.
• A discrete band of 2 kb was detected upon hybridization with a human beta-actin cDNA probe in Northern Blot analysis.
• Primers were designed to detect mRNA for genes of interest to confirm the phenotype of the cells.