cDNA
| Introduction to cDNA |
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BioChain's PCR Ready First Strand cDNA is an excellent source of tissue specific, PCR-ready, first strand cDNA. It can be immediately used for gene discovery or expression analysis. BioChain provides the largest selection of tissue cDNAs on the research market, this product line covers over 358 cDNAs from human adult and fetal normal tissues, human diseased and tumor tissues, rat, mouse, monkey and plant tissues.
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Features
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Ready to use for PCR
Oligo dT primer used to ensure the entire 3' end of cDNA is present
With some cDNA used as templates, 12 kb PCR amplicon was obtained to ensure the intact cDNAs
The largest selection of cDNAs from different tissues on the market
Documentation of tissues' clinical histories available
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| Applications |
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Immediate PCR Amplification of known genes
Verification of genetic mutation
Comparison of a specific gene between different tissues
Analysis of mRNA alternative splicing
Gene cloning and target sequencing
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| Description |
| First-Strand cDNA is synthesized from RNA isolated from a wide variety of documented human adult and fetel normal tissues, human diseased and tumor tissues, mouse, rat, monkey and plant tissues. Total RNA used for cDNA synthesis is isolated by modified guanidine thiocyanate techniques. 11 µg total RNA was primed by an oligo dT primer and reverse transcribed by MMLV reverse transcriptase in 40 µl final volume. RT reaction was stopped by heatiµg at 65°C for 10 minutes. The cDNA is delivered in 1x RT buffer. (1x RT Buffer: 50 mM Tris-Cl, pH 8.3, 75 mM KCl, 3 mM MgCl2, 10 mM DTT). 1 µl cDNA is sufficient for one PCR reaction. The 5' end of human clathrin cDNA (a 6 kb gene) has been amplified by PCR from all of these cDNAs. |
| Storage Conditions |
| Store cDNAs at -20°C |
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