Small Size Genomic DNA
| Introduction to Small Size Genomic DNA |
| PCR-based methods for the analysis of genomic DNA are becoming increasingly common both in research and for routine purposes. Genomic DNA on an average are between 50 - 150 kb, when the large molecule genomic DNA is used as PCR template, it might not be distributed evenly in solution when a master mix need to be made for multiple PCR reactions. High Throughput Digital PCR is a newly developed technology for high throughput real time PCR reaction, making master mix is inevitable in this technology. It is very crucial to make every molecule evenly distributed into each capilary chamber on the dynamic arrays. Large size genomic DNA molecules will result in uneven distribution while smaller size genomic will be even and randomly distributed. BioChain's small size genomic DNA will help you perform digital PCR successfully. More... |
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| Description |
| BioChain's small size genomic DNAs are isolated from a wide variety of tissues. The genomic DNAs are isolated from tissues by proprietary modified guanidine thiocyanate techniques. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. These genomic DNAs can be used as PCR template and have better performance in digital PCR comparing with genearl large size genomic DNA. A260/280 is between 1.8 and 2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination by RNA, polysaccharides and proteoglycans has been effectively eliminated. |
| Storage Conditions |
| Store genomic DNA in TE buffer at 2-8°C |
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