Universal cDNA
| Introduction to Universal cDNA |
| Universal cDNA is prepared by reverse transcribed BioChain's Universal RNA by random hexamer or oligo dT primer. Total RNA is isolated by modified guanidine thiocyanate techniques. The Universal cDNA can be used as controls for real time PCR and regular PCR, and also as a gene pools for cloning genes. BioChain's Universal cDNA is prepared with various diversified organs to ensure the broadest coverage of genes. Our Universal cDNA is made on an industrial scale to minimize the lot-to-lot variation. More... |
| Features (View Images) |
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| Description |
| 11 µg total RNA was primed by an olµgo dT primer or random hexamer and reverse transcribed by MMLV reverse transcriptase in 40 µl final volume. RT Reaction stopped by heatiµg at 65°C for 10 minutes. The cDNA is in 1x RT buffer. (1x RT Buffer: 50 mM Tris-CI, pH 8.3, 75 mM KCI, 3 mM MgCI2, 10 mM DTT). The estimated cDNA concentration is about 2.5 ng/µl. 1 µl cDNA is good enough for one PCR reaction. |
| Storage Conditions |
| Store Universal cDNA in storage solution at -20°C |
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Universal cDNA Reverse Transcribed by Oligo dT Primer: Chicken Normal Tissues
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100 rxn Läs mer -
Universal cDNA Reverse Transcribed by Oligo dT Primer: Dog Normal Tissues
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100 rxn Läs mer -
Universal cDNA Reverse Transcribed by Oligo dT Primer: Human Normal Tissues
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100 rxn Läs mer -
Universal cDNA Reverse Transcribed by Oligo dT Primer: Monkey Normal Tissues
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100 rxn Läs mer -
Universal cDNA Reverse Transcribed by Oligo dT Primer: Mouse Normal Tissues
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100 rxn Läs mer -
Universal cDNA Reverse Transcribed by Oligo dT Primer: Rat Normal Tissues
Beställningsvara
100 rxn Läs mer -
Universal cDNA Reverse Transcribed by Random Hexamer: Chicken Normal Tissues
Beställningsvara
100 rxn Läs mer -
Universal cDNA Reverse Transcribed by Random Hexamer: Dog Normal Tissues
Beställningsvara
100 rxn Läs mer -
Universal cDNA Reverse Transcribed by Random Hexamer: Human Normal Tissues
Beställningsvara
100 rxn Läs mer -
Universal cDNA Reverse Transcribed by Random Hexamer: Monkey Normal Tissues
Beställningsvara
100 rxn Läs mer -
Universal cDNA Reverse Transcribed by Random Hexamer: Mouse Normal Tissues
Beställningsvara
100 rxn Läs mer -
Universal cDNA Reverse Transcribed by Random Hexamer: Rat Normal Tissues
Beställningsvara
100 rxn Läs mer
12 artiklar
per sida